Golgi-specific or ER-specific biotin labeling proteome of the fat body by turboID during wing disc repair in Drosophila larvae
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ABSTRACT: Larval fat body samples were collected from 15-20 Gnmt-T2A>V5-turboID, Gnmt-T2A>Mgat2-V5-turboID, or Gnmt-T2A>ER-myc-turboID larvae in three biological replicates. Samples were collected at the early stage of wing imaginal disc repair. Biotinylated protein purified with neutralavidin beads were subjected to trypsin-digestion. Digested-peptides were desalinated and purified using GL-tip SDB. Purified peptides were subjected to LC-MS/MS analysis.
ORGANISM(S): Drosophila Melanogaster (fruit Fly)
SUBMITTER: Masayuki Miura
PROVIDER: PXD054313 | JPOST Repository | Wed Jun 11 00:00:00 BST 2025
REPOSITORIES: jPOST
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