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MBEC4 cells with low-Arginine conditions

ABSTRACT: Amino acid transporters are expressed in the brain capillary endothelial cells, which form the blood-brain barrier, and their expression levels change during the neonatal period. This study aimed to investigate the molecular mechanisms regulating the amino acid transporter levels in mouse brain capillary endothelial cells. Proteomic analysis revealed arginine depletion-dependent induction of the amino acid transporters, Slc1a4, Slc3a2, Slc7a1, Slc7a5, and Slc38a1. These effects were also inhibited by GCN2iB, suggesting the involvement of eIF2K4 activation. In contrast, expression levels of Slc2a1, Slc16a1, Abcb1b, Abcg2, transferrin receptor, insulin receptor, claudin-1, Zo-1, and Jam1 were not suppressed by GCN2iB.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Sumio Ohtsuki

PROVIDER: PXD054477 | JPOST Repository | Fri Apr 11 00:00:00 BST 2025

REPOSITORIES: jPOST

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			Action	DRS
	DMSO_Control_1.wiff	Wiff
	DMSO_Control_1.wiff.scan	Wiff
	DMSO_Control_1.wiff2	Wiff
	DMSO_Control_2.wiff	Wiff
	DMSO_Control_2.wiff.scan	Wiff

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Publications

Regulatory Role of eIF2αK4 in Amino Acid Transporter Expression in Mouse Brain Capillary Endothelial Cells.

Hamada Yudai Y Masuda Takeshi T Ito Shingo S Ohtsuki Sumio S

Pharmaceutical research 20241112 11

<h4>Purpose</h4>Amino acid transporters are expressed in the brain capillary endothelial cells that form the blood-brain barrier (BBB), and their expression levels change during the neonatal period. This study aimed to investigate the molecular mechanisms regulating amino acid transporter levels in mouse brain capillary endothelial cells.<h4>Methods</h4>Capillaries were isolated from the brains of neonatal and adult mice. Activation of eukaryotic translation initiation factor 2α kinase 4 (eIF2αK ...[more]

PMID: 39532778

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