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Visible light-mediated photocatalytic reaction between tetrazoles and carboxylic acids for protein and cell labelling

ABSTRACT: A photocatalytic reaction from tetrazole to nitrile imine, which can be coupled with carboxylic acids in aqueous solvents, has been developed. The reaction has been successfully used for photocatalyst-dependent labeling of carboxylic acids in proteins.

ORGANISM(S): Oryctolagus Cuniculus Schistosoma Japonicum Bos Taurus

SUBMITTER: Shinsuke Inuki

PROVIDER: PXD054950 | JPOST Repository | Tue Apr 01 00:00:00 BST 2025

REPOSITORIES: jPOST

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			Action	DRS
	240402ko02_Control.pepXML	Pepxml
	240402ko02_Control.raw	Raw
	240402ko02_Control_calibrated.mzML	Mzml
	240402ko03_143.pepXML	Pepxml
	240402ko03_143.raw	Raw

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Project description:Local adaptation and its underlying molecular basis has long been a key focus in evolutionary biology. There has recently been increased interest in the evolutionary role of plasticity and the molecular mechanisms underlying local adaptation. Using transcriptome analysis, we assessed differences in gene expression profiles for three brown trout (Salmo trutta) populations, one resident and two anadromous, experiencing different temperature regimes in the wild. The study was based on an F2 generation raised in a common garden setting. A previous study of the F1 generation revealed different reaction norms and significantly higher QST than FST among populations for two early life-history traits. In the present study we investigated if similar reaction norm patterns were present at the transcriptome level. Eggs from the three populations were incubated at two temperatures (5 and 8 degrees C) representing conditions encountered in the local environments. Global gene expression for fry at the stage of first feeding was analysed using a 32k cDNA microarray. The results revealed differences in gene expression between populations and temperatures and population × temperature interactions, the latter indicating locally adapted reaction norms. Moreover, the reaction norms paralleled those observed previously at early life-history traits. We were able to identify 90 cDNA clones among the genes with an interaction effect that were differently expressed between the ecologically divergent populations. These included genes involved in immune- and stress response. We observed less plasticity in the resident as compared to the anadromous populations, possibly reflecting that the degree of environmental heterogeneity encountered by individuals throughout their life cycle will select for variable level of phenotypic plasticity at the transcriptome level. Our study demonstrates the usefulness of transcriptome approaches to identify genes with different temperature reaction norms. The responses observed suggest that populations may vary in their susceptibility to climate change.

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Visible light-mediated photocatalytic reaction between tetrazoles and carboxylic acids for protein and cell labelling

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