Proteomics

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Golgi-specific or ER-specific biotin labeling proteome of the hemolymph by turboID during wing disc repair in Drosophila larvae


ABSTRACT: Larval hemolymph samples were collected from 12-20 Gnmt-T2A>V5-turboID, Gnmt-T2A>Mgat2-V5-turboID, or Gnmt-T2A>ER-myc-BirAG3ER larvae in three biological replicates. Samples were collected at the early stage of wing imaginal disc repair. Biotinylated protein purified with neutralavidin beads were subjected to trypsin-digestion. Digested-peptides were desalinated and purified using GL-tip SDB. Purified peptides were subjected to LC-MS/MS analysis.

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Masayuki Miura 

PROVIDER: PXD055017 | JPOST Repository | Wed Aug 20 00:00:00 BST 2025

REPOSITORIES: jPOST

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