Dataset Information

PEPPI-SP3: Robust and Simple Sample Prefractionation Workflow for Top-Down Proteomics

ABSTRACT: In this study, we developed a complete, robust, and simple sample preparation workflow named PEPPI-SP3 for top-down proteomics by combining PEPPI-MS with the magnetic bead-based protein purification approach used in SP3 (single-pot, solid-phase-enhanced sample preparation), now one of the standard sample preparation methods in bottom-up proteomics.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Nobuaki Takemori

PROVIDER: PXD056413 | JPOST Repository | Thu Jan 23 00:00:00 GMT 2025

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	240720_DDA17_Slot1-76_1_3418.d.zip	Other
	240720_DDA1_Slot1-73_1_3415.d.zip	Other
	240720_DDA21_Slot1-77_1_3419.d.zip	Other
	240720_DDA25_Slot1-78_1_3420.d.zip	Other
	240720_DDA33_Slot1-79_1_3421.d.zip	Other

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Publications

Gel-Based Sample Fractionation with SP3-Purification for Top-Down Proteomics.

Takemori Ayako A Sugiyama Naoyuki N Kline Jake T JT Fornelli Luca L Takemori Nobuaki N

Journal of proteome research 20250122 2

Precise prefractionation of proteome samples is a potent method for realizing in-depth analysis in top-down proteomics. PEPPI-MS (Passively Eluting Proteins from Polyacrylamide gels as Intact species for MS), a gel-based sample fractionation method, enables high-resolution proteome fractionation based on molecular weight by highly efficient extraction of proteins from polyacrylamide gels after SDS-PAGE separation. Thereafter it is essential to effectively remove contaminants such as CBB and SDS ...[more]

PMID: 39841590

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