Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

PSKH1:Reticulocalbin 3 (RCN3) SDA crosslinking

ABSTRACT: Recombinant proteins were mixed and then crosslinked by SDA and UV exposure. Complexes were resolved by SDS-PAGE, excised and tryptic peptides identified by MS.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Prof. James Murphy

PROVIDER: PXD056478 | JPOST Repository | Thu Jan 30 00:00:00 GMT 2025

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	CamKK_RCN3.fasta	Fasta
	P4866_02.mgf	Mgf
	P4866_02.raw	Raw
	P4866_03.mgf	Mgf
	P4866_03.raw	Raw

Items per page:

1 - 5 of 11

Similar Datasets

PSKH1:UNC119B SDA crosslinking

Project description:Recombinant proteins were mixed and then crosslinked by SDA and UV exposure. Complexes were resolved by SDS-PAGE, excised and tryptic peptides identified by MS.

2025-01-30 | PXD056476 | JPOST Repository

PSKH1:Calmodulin SDA crosslinking

Project description:Recombinant proteins were mixed and then crosslinked by SDA and UV exposure. Complexes were resolved by SDS-PAGE, excised and tryptic peptides identified by MS

2025-01-30 | PXD056468 | JPOST Repository

Pfs230 and Pfs48/45 complex SDA XL-MS

Project description:Asexual P. falciparum parasite cultures were maintained, gametocytes harvested and pellets lysed prior to chromatography procedures including size exclusion. Chemical cross-linking MS (XL-MS) analysis using SDA and UV exposure was performed followed by SDS-PAGE, gel bands excised and subjected to trypsin digestion and MS analysis.

2026-01-27 | PXD060341 | JPOST Repository

UV-crosslinking and high throughput sequencing of cDNAs (CRAC) of the RNA chaperone Hfq in enterohaemorhaggic E. coli

Project description:UV-crosslinking and high througput sequencing of cDNAs (CRAC) was used to map the binding sites Hfq in enterohaemorhaggic E. coli (EHEC). Hfq was tagged with a His-FLAG dual affintiy tag and UV crosslinked after growth in the MEM-HEPES media essentailly as per Granneman et al (2009) PNAS. We additionally crosslinked Hfq in non-pathogenic E. coli K12 str. MG1655 grown in LE media. Hfq-RNA complexes were purified and trimmed using RNase A/T1. RNA fragments were isolated and converted to cDNA, PCR amplified and sequenced using Illumina Solexa GAxII and HiSeq2000 platforms. His-FLAG tagged Hfq and untagged controls were cultured to an OD of 0.8 and crosslinked with UV-C. Five replicates of tagged EHEC Hfq and 2 replicates of untagged Hfq were crosslinked. We have additionally crosslinked 2 replicates each of E. coli K12 tagged and untagged Hfq.

2014-05-22 | E-GEOD-46118 | biostudies-arrayexpress

UV-crosslinking and high throughput sequencing of cDNAs (CRAC) of the RNA chaperone Hfq in enterohaemorhaggic E. coli

2014-05-22 | GSE46118 | GEO

UPA-Seq: Prediction of functional lncRNAs using the sensitivities to UV-crosslinking

Project description:Assuming that functional lncRNAs form larger ribonucleoprotein complex and thus are easily crosslinked to proteins upon UV-irradiation, we performed RNA-Seq analyses of RNAs recovered from the aqueous phase after the UV-irradiation and phenol chloroform extraction (UPA-Seq)

2018-09-21 | GSE114789 | GEO

PINK1-TOM-TIM23 complex SDA XL-MS

Project description:3xFLAG-tagged human wild-type PINK1 was expressed and immunoprecipitated with anti-FLAG beads. Chemical cross-linking MS (XL-MS) analysis using SDA and UV exposure was performed followed by in solution trypsin digestion and MS analysis.

2025-05-29 | PXD058526 | JPOST Repository

Experiments using TurboID and BPA crosslinking to identify interactions between SNIPE, ManYZ, and phage proteins

Project description:TurboID was fused to different inner membrane proteins and proximity labeling + streptavidin bead pulldowns were used to identify biotinylated proteins. Separately, BPA was incorporated into SNIPE-FLAG and crosslinked to phage and host proteins with UV exposure, followed by anti-FLAG pulldowns and mass spectrometry to identify crosslinked proteins.

2025-03-11 | MSV000097285 | MassIVE

Penicillium sp. MCCC001 isolate:SDA

Project description:Penicillium sp. MCCC001 isolate:SDA Genome sequencing

| PRJNA565898 | ENA

Transcription profiling of rice plants under the Sda (Air-drying) condition in shoots

Project description:In this series of experiments, we wanted to study the transcriptional responses of plants to different levels of water limitation. For mild drought stress, we controlled water potential (a scientific concept for dryness of soil) by using an automated watering system. This system adds water to soil based on the pF values reported by a pF sensor in soil. pF is a classical and widely used index of water potential that was first defined by Schofield (1935). For severe stress conditions, we withheld watering or even dried plants on a lab bench. Here, the transcriptional profiles were compared between well-watered and Sda-treated rice seedling shoots.<br>Sda (Severe dehydration stress by air drying): We designated the Sda as a term that means a dehydration stress in our experiments. The dehydration stress treatment was carried out by pulling plants from the soil and leaving them on a bench.

2016-12-26 | E-MTAB-4137 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data