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FLAG-IP-MS analyses of novel oxidative stress responsive translation factors

ABSTRACT: We identified translation-related factors with significantly elevated cysteine oxidation upon MND treatment by our redox proteomics method DIALRP. Notably, our data demonstrate that the inhibition of such as EIF2, EIF6, and EEF2 complex formation due to oxidative stress occurs during the cellular response of translational inhibition.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Masaki Matsumoto

PROVIDER: PXD057922 | JPOST Repository | Thu Jul 31 00:00:00 BST 2025

REPOSITORIES: jPOST

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			Action	DRS
	20240903_HeLa_EF2_FLAGIP_DMSO_1_050.raw	Raw
	20240903_HeLa_EF2_FLAGIP_DMSO_3_050.raw	Raw
	20240903_HeLa_EF2_FLAGIP_Mena_1_050.raw	Raw
	20240903_HeLa_EF2_FLAGIP_Mena_2_050.raw	Raw
	20240903_HeLa_EF2_FLAGIP_Mena_3_050.raw	Raw

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Publications

Data-Independent Acquisition (DIA)-Based Label-Free Redox Proteomics (DIALRP) Identifies Prominent Cysteine Oxidations in Translation Machinery in Prostate Cancer Cells Under Oxidative Stress.

Kobayashi Daiki D Takami Tomoyo T Matsumoto Masaki M

Journal of proteome research 20250731 9

Oxidative stress is a key factor in numerous physiological and pathological processes, including aging, cancer, and neurodegenerative diseases. Protein cysteine residues are particularly susceptible to oxidative stress-induced modifications that can alter their structure and function, thereby affecting intracellular signaling pathways. In this study, we performed a data-independent acquisition mass spectrometry (DIA-MS)-based label-free redox proteomics method, termed DIALRP, to comprehensively ...[more]

PMID: 40740030

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