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Proteome of BCG-derived membrane vesicles

ABSTRACT: Proteins in two different BCG-derived membrane vesicles, extracellular vesicles, were compared. One is isolated from statically cultured BCG in Sauton's medium (Sample #1 and #2), commonly used to produce BCG vaccine. Another is isolated from planktonically cultured BCG in Muller-Hinton II Broth (Sample #3 and #4), commonly used for bacterial experiments. Proteins from BCG-MVs were separated via SDS-PAGE on a 12% polyacrylamide gel (10 µg in each lane). The resulting gel was stained with 10% Coomassie Brilliant Blue (G-250), and 4 pieces per lane were excised. The samples were reduced, alkylated and trypsinized via the in-gel digestion method. The peptides extracted from the gel were subjected to nanoLC-MS/MS analysis with a system consisting of an Orbitrap Velos Pro (Thermo) coupled with an Advance UHPLC (Brucker).

ORGANISM(S): Mycobacterium Tuberculosis Variant Bovis Bcg

SUBMITTER: Takehiro Yamaguchi

PROVIDER: PXD058214 | JPOST Repository | Tue Nov 25 00:00:00 GMT 2025

REPOSITORIES: jPOST

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			Action	DRS
	F089344.dat	Other
	F089345.dat	Other
	F089346.dat	Other
	F089347.dat	Other
	orb_200124_BS-TY-1_%231-1.raw	Raw

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