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MS for 10,000 g pellet fraction of Caki1, ACHN, LNCaP and HEK293T

ABSTRACT: The 10,000 g pellet fraction of the renal cancer cell line Caki1 and ACHN conditioned medium (CM) induced PSMA-positivity in human umbilical vein endothelial cells (HUVECs) and en-hanced tube formation. Mass spectrometry indicated that the 10,000 g pellet fraction contained various kinds of growth factors.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Ryuta Watanabe

PROVIDER: PXD058876 | JPOST Repository | Mon Dec 15 00:00:00 GMT 2025

REPOSITORIES: jPOST

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			Action	DRS
	%C3%A7%C2%B9%C2%A7%C3%AF%C2%BD%C2%BF%C3%A7%C2%B9%C2%9D%C3%AF%C2%BD%C2%B3%C3%A7%C2%B9%C2%9D%C3%A4%C2%B8%C5%A0%C3%AF%C2%BD%C5%A1%C3%A7%C2%B9%C2%A7%C3%AF%C2%BD%C2%AF%C3%A9%E2%80%BA%E2%80%B0%C3%AF%C2%BD%C2%AA%C3%A8%C5%93%C2%B7%C3%A6%E2%80%9A%C5%B8%C3%AF%C2%BD%C2%AE%C3%A5%C2%A4%E2%80%9A%C3%AF%C2%BD%C2%B5%C3%A5%E2%80%A6%CB%86%C3%A6%C2%A3%C2%A1%C3%A7%C2%B8%C2%BA%C3%AF%C2%BD%C2%AB%C3%A7%C2%B8%C2%BA%C3%AF%C2%BD%C2%A4%C3%A7%C2%B8%C2%BA%C3%A3%C6%92%C2%BB%C3%A2%E2%82%AC%C2%BB.pdf	Pdf
	MS_CM.xlsx	Xlsx

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Project description:Abstract: Background & Aims: Unusual hypervascularity is a hallmark of human hepatocellular carcinoma (HCC). Although microRNA-214 (miR-214) is upregulated in other human cancers, it is downregulated in HCC. We elucidated the biological and clinical significance of miR-214 downregulation in HCC. Methods: MicroRNAs deregulated in HCC were identified using array-based MicroRNA profiling. A luciferase reporter assay confirmed target association between miR-214 and hepatoma-derived growth factor (HDGF). Tube formation and in vivo angiogenesis assays validated the roles of miR-214/HDGF in angiogenesis. Results: MiR-214 downregulation was associated with higher tumor recurrence and worse clinical outcomes. Ectopic expression of miR-214 suppressed xenograft tumor growth and microvascularity of the tumor and its surrounding tissues. The genes downregulated by ectopic expression of miR-214 were involved in the regulation of apoptosis, cell cycle, and angiogenesis. Integrated analysis disclosed HDGF as a downstream target of miR-214. Conditioned medium of HCC cells contained bioactivity to stimulate tube formation of human umbilical vein endothelial cells, which was abolished by pretreatment of the conditioned media with HDGF antibodies, silencing of HDGF expression or ectopic expression of miR-214 in the donor HCC cells. The angiogenic activity of the conditioned media lost by ectopic expression of miR-214 in the donor cells was restored by supplementation with recombinant HDGF. In vivo tumor angiogenesis assays showed significant suppression of tumor vascularity by ectopic expression of miR-214. Conclusions: A novel role of microRNA in tumrigenesis is identified. Downregulation of miR-214 contributes to unusual hypervascularity of HCC via activation of the HDGF paracrine pathway for tumor angiogenesis. To identify miRNAs that are deregulated in human HCC, 68 HCC and 21 non-tumor liver tissues were subjected to profiling of miRNA expression using miRNA arrays containing 739 human miRNA probes. Differentially expressed microRNAs were identified.

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MS for 10,000 g pellet fraction of Caki1, ACHN, LNCaP and HEK293T

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