Structural Insights into the Role of Eukaryotic Translation Initiation Factor 3 (eIF3) in Translation Mediated by the Hepatitis C Virus IRES
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ABSTRACT: Cross-linking mass spectrometry was conducted to determine the positions of the non-core subunits (eIF3b, eIF3d, eIF3g, eIF3i, and eIF3j) of eIF3, that were not observed in cryo-EM densities, during elongation. For this analysis, the HCV IRES-initiated uORF2-stalled 80S ribosome was prepared from the in vitro translation system coupled with transcription, identical to the preparation used for the cryo-EM sample.
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Takuhiro Ito
PROVIDER: PXD059272 | JPOST Repository | Thu Nov 20 00:00:00 GMT 2025
REPOSITORIES: jPOST
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