Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Blood-brain barrier proteomics

ABSTRACT: Brain microvessels (BMVs) were isolated from frozen human and mouse cerebral cortices, as well as frozen mouse cerebrums. BMVs and cultured brain microvascular endothelial cells (BMECs), such as hCMEC/D3, HBMEC/ci, and primary BMECs, were analyzed via data-independent acquisition using liquid chromatography-mass spectrometry.

ORGANISM(S): Homo Sapiens (human) Cellular Organisms Mus Musculus (mouse)

SUBMITTER: Sumio Ohtsuki

PROVIDER: PXD060008 | JPOST Repository | Wed Jul 23 00:00:00 BST 2025

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20220510_Masuda_1ug_Easy_0202Nikkyo_120min_DIA_VarWid_StepCE3_D3_1.raw	Raw
	20220510_Masuda_1ug_Easy_0202Nikkyo_120min_DIA_VarWid_StepCE3_D3_2.raw	Raw
	20220510_Masuda_1ug_Easy_0202Nikkyo_120min_DIA_VarWid_StepCE3_D3_3.raw	Raw
	20220510_Masuda_1ug_Easy_0202Nikkyo_120min_DIA_VarWid_StepCE3_HUVEC_1.raw	Raw
	20220510_Masuda_1ug_Easy_0202Nikkyo_120min_DIA_VarWid_StepCE3_HUVEC_2.raw	Raw

Items per page:

1 - 5 of 20

Publications

Proteome profile differences among human, monkey, and mouse brain microvessels and cultured brain microvascular endothelial cells.

Kumabe Haruka H Masuda Takeshi T Ito Shingo S Furihata Tomomi T Toda Akiko A Mogi Masayuki M Araki Norie N Ohtsuki Sumio S

Fluids and barriers of the CNS 20250530 1

<h4>Background</h4>The blood-brain barrier (BBB) expresses transporters, receptors, and tight junction proteins that regulate the exchange of substances between the blood and brain. The differences in the expression of these proteins in the BBB among different species and cultured BBB model cells should be clarified to interpret the BBB function in model animals and cells. This study aimed to elucidate species differences among humans, monkeys, and mice and in vitro-in vivo differences in the BB ...[more]

PMID: 40448242

Similar Datasets

MiR-155 promotes blood-brain barrier dysfunction in neuroinflammation (part 1)

Project description:Here, we investigated the time-course changes in the pattern of microRNA (miRNA) expression of TNFα and IFNγ-stimulated and unstimulated hCMEC/D3 cells, an immortalized human cerebral microvascular endothelial cell line. In order to investigate pro-inflammatory cytokine-induced changes in miRNA levels in hCMEC/D3 cells, we challenged brain endothelial cells with TNFα and IFNγ (100 ng/ml) for 2 h, 6 h and 24 h and determined microRNA expression in cytokine-stimulated and unstimulated cells

2014-04-29 | E-GEOD-44692 | biostudies-arrayexpress

Effects of Smoldering Eucalyptus Wood Smoke Extract on Primary Human Brain Microvascular Endothelial Cells (HBMEC) and Immortalized Human Cerebral Microvascular Endothelial Cell Line (hCMEC/D3)

Project description:Increase in frequency and size of wildfire raises a significant public health concern due to rapid climate change. Epidemiological studies have shown that wildfire smoke exposure can increase the risk of neurological disorders. Our study aims to understand how wildfire smoke particles affect brain endothelial cells using both primary human brain microvascular endothelial cells (HBMEC) and an immortalized human cerebral microvascular endothelial cell line (hCMEC/D3). HBMEC and hCMEC/D3 were exposed to different levels of smoldering eucalyptus smoke particles (10, 30, 50 ug/mL) for 24 hr. Supernatants were collected for LDH and ELISA array, cell lysates were collected for RNAseq, and cells were fixed and stained for tight junction marker (Zonula Occludens-1, ZO-1) via ICC. Our study found that treatment to smoldering eucalyptus particles can increase inflammatory cytokine production dose-dependently in HBMECs and hCMEC/D3. Moreover, RNAseq analyses resulted in dose-dependent changes in HBMEC and hCMEC/D3 from wood smoke treatment that could be mediated via AhR and Nrf2 pathways.

2024-10-02 | GSE271935 | GEO

Human Blood Brain Barrier treated by Insulin

Project description:This study aims to map the dynamics of insulin-responsive pathways in polarized human cerebral microvascular endothelial cell (hCMEC/D3) monolayers, a widely used BBB cell culture model, to elucidate molecular mechanisms underlying BBB dysfunction in AD.

2022-02-05 | GSE195781 | GEO

MiR-155 promotes blood-brain barrier dysfunction in neuroinflammation (part 1)

2014-04-29 | GSE44692 | GEO

MiR-155 promotes blood-brain barrier dysfunction in neuroinflammation (part 2)

Project description:Over-expression of miR-155 induces changes in the pattern of gene expression of hCMEC/D3 cells. hypothesis tested in the present study was that miR-155 constitute an important regulatory control of the brain endothelial response to inflammatory cytokines. To identify miR-155 target genes in brain endothelim that might be implicated in BBB dysfunction relevant to human disease, we then analysed changes in mRNA expression of hCMEC/D3 cells that overexpress miR-155 and results were contrasted to cells transfected with scrambled miR. To ectopically express miR-155 in hCMEC/D3 cells, 30 nM of pre-miR-155 and the siPORT Amine transfection agent (Applied Biosystems, Warrington, UK) were combined following the manufacturerM-bM-^@M-^Ys instructions.

2014-04-29 | E-GEOD-44693 | biostudies-arrayexpress

BBBomics - Human Blood Brain Barrier Transcriptomics Hub [RNA-seq]

Project description:Functional and structural dysfunction of the blood brain barrier (BBB) leads to severe alterations in brain physiology and is believed to trigger neurodegeneration. To investigate the molecular mechanisms driving the BBB dysfunction, very few human BBB cell culture models are available; of which, the human microvascular endothelial cell line (hCMEC/D3) is the most widely used. Thus far, array-based approaches or targeted seqeuncing based approaches have been employed to characterize the gene expression of the hCMEC/D3 model. However,The goal of this study is to perform deep transcriptomic sequencing of the BBB cell line and obtain features like gene expression, expressed single nucleotide variants, alternate splice forms, circular RNAs, long non-coding RNAs and micro RNAs. We have developed blood brain barriers transcriptomics landscape using RNA sequencing and micro RNA seqeuncing data obtained from replicates of hCMEC/D3 BBB cell line.

2016-03-17 | E-GEOD-76528 | biostudies-arrayexpress

BBBomics - Human Blood Brain Barrier Transcriptomics Hub [miRNA-seq]

Project description:Functional and structural dysfunction of the blood brain barrier (BBB) leads to severe alterations in brain physiology and is believed to trigger neurodegeneration. To investigate the molecular mechanisms driving the BBB dysfunction, very few human BBB cell culture models are available;of which, the human microvascular endothelial cell line (hCMEC/D3) is the most widely used. Thus far, array-based approaches or targeted seqeuncing based approaches have been employed to characterize the gene expression of the hCMEC/D3 model. However,The goal of this study is to perform deep transcriptomic sequencing of the BBB cell line and obtain features like gene expression, expressed single nucleotide variants, alternate splice forms, circular RNAs, long non-coding RNAs and micro RNAs. We have developed blood brain barriers transcriptomics landscape using RNA and micro RNA sequencing data obtained from replicates of hCMEC/D3 BBB cell line.

2016-03-17 | E-GEOD-76530 | biostudies-arrayexpress

Neutrophil-derived microvesicle induced dysfunction of the blood-brain barrier in vitro

Project description:The aim of this study is to understand how NMV can affect the vascular integrity of the blood-brain barrier using a cell line- hCMEC/D3. Recent studies have reported that NMV can affect other endothelial barriers in the body contributing to inflammation and pathogenesis of diseases such as atherosclerosis. The transcriptomic profile of NMV-treated hCMEC/D3 cells was compared to that of cells without treatment to understand whether functional groupings and pathways are differentially expressed, which can contribute to changes in blood-brain barrier.

2019-12-05 | GSE137111 | GEO

MiR-155 promotes blood-brain barrier dysfunction in neuroinflammation (part 2)

2014-04-29 | GSE44693 | GEO

BBBomics - Human Blood Brain Barrier Transcriptomics Hub [RNA-seq]

2016-03-17 | GSE76528 | GEO