Dataset Information

FFPE proteome analysis of non-cancer lesion of renal cell carcinoma

ABSTRACT: To extract the molecules that correlate with kidney function and fibrosis, we performed the proteome analysis of non-cancer lesion of renal cell carcinoma in 16 patients. Proteins were extracted from FFPE slice samples and digested using the SP3 method as previously described. The digested peptide samples were analyzed by DIA-MS on a 6600 TripleTOF interfaced to Eksigent NanoLC400 system. Protein identification and quantification were performed using the library-free search function DIA-NN 1.9.2, with the UniProt human reference proteome allowing one miscleavage. The intensities of the precursors were normalized using retention time-dependent cross-run normalization. Peptides and proteins were filtered at a false discovery rate of < 1% for identification and quantification.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Sumio Ohtsuki

PROVIDER: PXD060453 | JPOST Repository | Tue Oct 21 00:00:00 BST 2025

REPOSITORIES: jPOST

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Publications

Fibroblast-specific palladin drives kidney fibrosis via MRTF-SRF signaling.

Yamamoto Naoki N Sakai Norihiko N Yamamura Yuta Y Kaikoi Daichi D Hayashi Daiki D Matsuno Takahiro T Koshino Akihiko A Sako Keisuke K Horikoshi Keisuke K Yuasa Takahiro T Tamai Akira A Minami Taichiro T Oshima Megumi M Nakagawa Shiori S Kitajima Shinji S Hara Akinori A Shimizu Miho M Terakawa Jumpei J Horike Shin-Ichi SI Daikoku Takiko T Mizokami Atsushi A Ikeda Hiroko H Kadoguchi Moeno M Arakawa Hiroshi H Ohtsuki Sumio S Lagares David D Wada Takashi T Iwata Yasunori Y

The Journal of pathology 20251024 1

Fibrosis is a common end-stage pathway of progressive chronic kidney diseases. Previously we demonstrated that myocardin-related transcription factor (MRTF)-serum response factor (SRF) signaling drives the expression of fibrosis-related molecules through actin cytoskeleton dynamics in renal fibroblasts. However, it has not been elucidated whether actin-associated proteins relate to the pathogenesis of fibrosis. Here, we reveal that the actin cytoskeleton-regulating pathway is significantly corre ...[more]

PMID: 41131992

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			Action	DRS
	K10.wiff	Wiff
	K10.wiff.scan	Wiff
	K11.wiff	Wiff
	K11.wiff.scan	Wiff
	K12.wiff	Wiff