Salmon nasal cartilage proteoglycan up-regulates Listeria monocytogenese-mediated immune response in mice

ABSTRACT: The proteomic analysis of liver samples from mice administered proteoglycan (PG) for 9 days or untreated control mice. Liver samples from mice administered PG for 9 days or untreated control mice (n = 6 per group) were homogenized in PBS. The proteins were precipitated using acetone. The supernatant from the homogenized liver was transferred into two-milliliter microcentrifuge tubes, and four times the sample volume of cold (-20oC) acetone was added. After the vortex, the tubes were incubated overnight at -20oC. Acetone was removed by centrifugation at 14,000 x g for 10 min at 4oC, then the remaining acetone was allowed to evaporate at room temperature for 30 min. The protein pellet was dissolved in PBS, and protein concentration was measured using Bradford protein assay. Proteins were first denatured using 50% trifluoroethanol and reduced with 4 mM dithiothreitol. Alkylation of free cysteine residues was performed before trypsin digestion, and the desalted peptides were subsequently separated through liquid chromatography. A mass spectrometer was used to analyze the samples. The resulting spectra were compared against Mus musculus proteins to assess differences in protein expression between PG-treated mice and non-treated controls (NC), determining the relative abundance of proteins in each group.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Krisana Asano 

PROVIDER: PXD061495 | JPOST Repository | Thu Mar 05 00:00:00 GMT 2026

REPOSITORIES: jPOST