Proteomics

Dataset Information

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Identification of the target protein of the anticancer peptide Yaku’amide B

ABSTRACT: Yaku’amide B-binding proteins were enriched and purified from MCF-7 cell lysates using a biotinylated photoaffinity probe of Yaku’amide B through photoaffinity labeling (PAL) pull-down, and subsequently identified by mass spectrometry.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Masayuki Inoue 

PROVIDER: PXD062001 | JPOST Repository | Thu Mar 12 00:00:00 GMT 2026

REPOSITORIES: jPOST

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SN210435_A_10ul.mzid Mzid
SN210435_A_10ul.mzid_SN210435_A_10ul.MGF Mzid
SN210435_A_10ul.raw Raw
SN210436_B_10ul.mzid Mzid
SN210436_B_10ul.mzid_SN210436_B_10ul.MGF Mzid
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Publications

Photoaffinity Labeling Strategy Reveals Tetraspanin CD9 as a Transient Target of Anticancer Yaku'amide B.

Fu Junhao J   Kamiya Koichi K   Kitamura Kai K   Kawahara Ryo R   Shihoya Wataru W   Nureki Osamu O   Hosoya Shoichi S   Nakagawa Reiko R   Mashima Tetsuo T   Itoh Hiroaki H   Inoue Masayuki M   Sakurai Kaori K  

Journal of the American Chemical Society 20260109 3

Innovative chemical strategies are needed to understand the molecular targets of natural products, especially when they engage with multiple targets via transient interactions. Yaku'amide B (<b>1</b>), an anticancer natural product, inhibits mitochondrial F<sub>o</sub>F<sub>1</sub>-ATP synthase with nanomolar affinity; however, this mechanism alone does not explain its unique activity. Here, we employed photoaffinity labeling (PAL), a powerful, chemical strategy, to discover that tetraspanin CD9  ...[more]

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