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Co-IP and MS analysis of LacZ or 3xHA:LUBEL-N

ABSTRACT: HA-LUBEL 1-872 or LacZ (control) was expressed under the control of Mef2-GAL4, and the progenies were raised at 25°C. The 3IL carcass fillet lysates were subjected to anti-HA immunoprecipitation. To identify potential binding partners, we performed immunoprecipitation-mass spectrometry (IP-MS) of LUBEL1-872.

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Fujita Naonobu

PROVIDER: PXD062558 | JPOST Repository | Sat Nov 22 00:00:00 GMT 2025

REPOSITORIES: jPOST

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			Action	DRS
	20220912_Yoshikawa_B_Fujita_2_NonNormalization.pdResult	Other
	20220912_Yoshikawa_B_Fujita_2_NonNormalization.xlsx	Xlsx
	239_Yoshikawa_B_Fujita_1.raw	Raw
	241_Yoshikawa_B_Fujita_2.raw	Raw
	243_Yoshikawa_B_Fujita_3.raw	Raw

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Publications

Linear ubiquitination triggers Amph-mediated T-tubule biogenesis.

Kawaguchi Kohei K Hama Yutaro Y Yoshikawa Harunori H Nishino Kohei K Morimoto Kazuki K Nakamura Tsuyoshi T Koizumi Michiko M Sakamaki Yuriko Y Abe Kota K Kakuta Soichiro S Ichimura Koichiro K Ikeda Fumiyo F Kosako Hidetaka H Fujita Naonobu N

Science advances 20260107 2

Transverse tubules (T-tubules) are invaginations of the muscle plasma membrane that facilitate rapid transmission of action potentials, ensuring synchronized muscle contraction. Despite their essential role in muscle physiology, the mechanisms underlying T-tubule formation remain elusive. Here, we identify LUBEL/RNF31, a ubiquitin E3 ligase responsible for linear (M1-linked) ubiquitination, as a key regulator of T-tubule biogenesis in <i>Drosophila</i>. Loss of LUBEL leads to Amphiphysin (Amph)- ...[more]

PMID: 41499502

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