Proximity proteomics of T-tubules using miniTurbo-fused LUBEL

ABSTRACT: To identify candidate genes unbiasedly, we employed biotinylation-based proximity proteomics of T-tubules in vivo. We generated a transgenic line carrying an LUBEL construct fused with miniTurbo (mnTb). We expressed either LUBEL-mnTb or mnTb alone in muscles, and larvae were reared in the presence or absence of biotin. As a control, LacZ was expressed instead of the mnTb fused constructs. Biotinylated peptides were extracted purified from trypsin-digested samples using Tamavidin 2-REV beads and analyzed by mass spectrometry.

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Fujita Naonobu 

PROVIDER: PXD062634 | JPOST Repository | Sat Nov 22 00:00:00 GMT 2025

REPOSITORIES: jPOST