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Storage Protein-Mediated Translation Control Links Juvenile Diet to Longevity in Drosophila

ABSTRACT: Dietary restriction (DR), whether applied during adulthood or juvenile stages, extends lifespan across diverse species. However, the mechanisms by which early-life dietary interventions influence adult physiology and longevity remain poorly understood. Here, using Drosophila as a model, we demonstrate that protein restriction during the larval stage (early-life protein restriction, ePR) promotes adult lifespan by reducing storage protein levels. Stable isotope tracing reveals that dietary amino acids obtained in the larval stage are retained into adulthood, especially incorporated into ribosomal proteins. This is mediated by larval serum protein 2 (Lsp2), a major storage protein, whose expression is durably downregulated by ePR. Both dietary (ePR) and genetic (Lsp2-RNAi) reduction of the protein storage lead to decreased ribosomal protein levels and translation activity in early adulthood. Notably, these storage proteins are enriched in aromatic amino acids such as tyrosine, and larval tyrosine restriction alone is sufficient to suppress translation and promote longevity. These findings show that storage proteins mediate the effect of larval nutrition on adult longevity via controlling translation. Our study uncovers a previously unrecognised mechanism of nutritional memory that links early-life diet to life-long organismal health.

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Koshi Imami

PROVIDER: PXD062930 | JPOST Repository | Fri Jul 17 00:00:00 BST 2026

REPOSITORIES: jPOST

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			Action	DRS
	20241227_QE_KI_146_DM_CantonS_Saf1_female_13.raw	Raw
	20241227_QE_KI_146_DM_CantonS_Saf1_female_14.raw	Raw
	20241227_QE_KI_146_DM_CantonS_Saf1_female_15.raw	Raw
	20241227_QE_KI_146_DM_CantonS_Saf1_male_20.raw	Raw
	20241227_QE_KI_146_DM_CantonS_Saf1_male_21.raw	Raw

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