Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteome data of human iPSC and original fibroblast (HDF)

ABSTRACT: Proteome data for human induced pluripotent stem cells (iPSCs, 201B7) and human dermal fibroblasts (HDFs, HDF1388) were acquired using a Bruker timsTOFpro. These mass spectrometry data were analyzed using DIA-NN 1.8.1.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Mio Iwasaki

PROVIDER: PXD063003 | JPOST Repository | Wed Apr 16 00:00:00 BST 2025

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	220516mi-06-Ff-201B7-whole2-n1-200ng-DIA_Slot1-01_1_8462.d.zip	Other
	220516mi-07-Ff-201B7-whole1-n2-200ng-DIA_Slot1-13_1_8463.d.zip	Other
	220516mi-08-Ff-201B7-whole3-n3-200ng-DIA_Slot1-25_1_8464.d.zip	Other
	220519mi-06-HDF1388-slc-whole1-n1-200ng-DIA_Slot1-01_1_8490.d.zip	Other
	220519mi-07-HDF1388-slc-whole-n2-200ng-DIA_Slot1-13_1_8491.d.zip	Other

Items per page:

1 - 5 of 7

Similar Datasets

Gene expression profiles of induced pluripotent stem cells from centenarians

Project description:We established induced pluripotent stem cells (iPSC) from centrenarians by retroviral transduction of primary human fibroblasts. To show the similarity between 201B7 iPSC and 100-1 #16 iPSC (induced pluripotent stem cells from centenarian), this experiment was designed. Undifferentiated 201B7 iPSC, and 100-1 #16 iPSCs were collected. Then, they were applied in this experiment.

2012-09-01 | E-GEOD-36667 | biostudies-arrayexpress

Gene expression profiles of induced pluripotent stem cells from centenarians

2012-09-01 | GSE36667 | GEO

Human dermal fibroblast reprogramming: compact and differentiated colonies during maturation phase

Project description:Transcriptional profiling of reprogrammed cells at day 20 post-transduction comparing compact and differentiated colonies with established induced Pluripotent Stem Cells (iPSC) generated from human dermal fibroblasts (HDF). This study focused in the identification of upregulated transcription factors related to early developmental processes, particularly during primitive streak formation.

2017-09-06 | GSE103491 | GEO

Gene expression profiles of familial Alzheimer's disease with presenilin 2 mutation patient-specific induced pluripotent stem cells

Project description:We established two clones of induced pluripotent stem cells (iPSC) with the presenilin 2 mutation, N141 (PS2-1 iPSC and PS2-2 iPSC) by retroviral transduction of primary human fibroblasts. To show the similarity among 201B7 iPSC, PD01-25 iPSC(Sporadic Parkinson's disease patient derived iPSC), PS2-1 iPSC, PS2-2 iPSC, this experiment was designed. Undifferentiated 201B7 iPSC, PD01-25 iPSC, PS2-1 iPSC and PS2-2 iPSC were collected. Then, they were applied in this experiment.

2011-09-09 | E-GEOD-28379 | biostudies-arrayexpress

Metabolomics Workbench ST000003 - GNPS - Metabolomic analysis of mouse embryonic fibroblasts, embryonic stem cells, and induced pluripotent stem cells

Project description:mouse embryonic fibroblasts, embryonic stem cells, and induced pluripotent stem cells were compared via metabolomic analysis

2018-08-02 | MSV000082680 | GNPS

Pluripotent reprogramming arises from a unique subset of pre-disposed human dermal fibroblasts

Project description:Induced cellular reprogramming to the pluripotent state offers a novel stem cell source for autologous transplantation. While recent studies have explored the role of factors required for induced pluripotent stem cell (iPSC) induction, the cellular and molecular basis of reprogramming from human fibroblasts remains elusive. Here, we have identified a subset of human dermal-derived fibroblasts that shares hallmark molecular and epigenetic features with pluripotent cells. Functional studies demonstrate that these cells contribute to the majority of human iPSCs generated from dermal fibroblasts and are dependent on heterogeneous fibroblast microenvironment for reprogramming competency. Molecular characterization indicated these predisposed fibroblasts were unique to other dermal derived stem cells and possessed features of proliferative selfrenewal. Our study reveals human fibroblasts are not equivalently capable of cellular reprogramming, and suggests that reprogramming factors overcome commitment steps that allow predetermined dermal fibroblasts to establish stable pluripotent state.

2021-11-01 | GSE20904 | GEO

Transcriptional analysis of pre and post excision human induced pluripotent stem cells

Project description:Transcriptional analysis was performed on pre and post excision human induced pluripotent stem cells, the donor human dermal fibroblasts (HDFs) they were derived from and control human embryonic stem cells We isolated total RNA from pre and post excision human induced pluripotent stem cells, the donor human dermal fibroblasts (HDFs) they were derived from and control human embryonic stem cells and analyzed via Affymetrix microarray analysis.

2013-07-13 | E-GEOD-48830 | biostudies-arrayexpress

sibling aHDF-iPSC clones derived from the same fibroblasts

Project description:Hepatocytes generated from human induced pluripotent stem cells (hiPSCs) are unprecedented resources for pharmaceuticals and cell therapy. However, little attention has so far been paid to variations among hiPSC lines in terms of their hepatic differentiation. We developed an improved hepatic differentiation protocol and compared multiple hiPSC lines. This comparison indicated that the hepatic differentiation propensity varies among sibling hiPSC clones derived from the same adult human dermal fibroblasts (aHDFs). In addition, hiPSC clones derived from peripheral blood cells (PB-iPSCs) consistently showed good hepatic differentiation efficiency, whereas many hiPSC clones from adult dermal fibroblasts (aHDF-iPSCs) showed poor hepatic differentiation. However, when we compared hiPSCs from blood and dermal fibroblasts from the same individuals, we found that variations in hepatic differentiation were largely attributable to donor differences, rather than to the types of the original cells. In order to understand the molecular mechanisms underlying the observed variations in hepatic differentiation, we performed microarray analyses of sibling aHDF-iPSC clones, and aHDF- and PB-iPSC clones from the same individuals. sibling aHDF-iPSC clones (201B6 and 201B7; derived from the same aHDFs) (1) undifferentiated state (n=4, biological replicate #1-#4) (2) CXCR4-positive cell populations sorted by flowcytometry after 7 days of endodermal differentiation (n=4, biological replicate #1-#4) (3) CXCR4-negative cell populations sorted by flowcytometry after 7 days of endodermal differentiation (n=4, biological replicate #1-#4)

2012-07-15 | E-GEOD-38152 | biostudies-arrayexpress

DNA Methylation profiling of human iNBSCs, iPSC-derived NBSCs and adult dermal fibroblasts

Project description:We describe a so far uncharacterized, embryonic and self-renewing Neural Plate Border Stem Cell (NBSC) population with the capacity to differentiate into central nervous and neural crest lineages. NBSCs can be obtained by neural transcription factor-mediated reprogramming (BRN2, SOX2, KLF4, and ZIC3) of human adult dermal fibroblasts and peripheral blood cells (induced Neural Plate Border Stem Cells, iNBSCs) or by directed differentiation from human induced pluripotent stem cells (NBSCs). Moreover, human (i)NBSCs share molecular and functional features with an endogenous NBSC population isolated from neural folds of E8.5 mouse embryos. Upon differentiation, iNBSCs give rise to either (1) radial glia-type stem cells, dopaminergic and serotonergic neurons, motoneurons, astrocytes, and oligodendrocytes or (2) cells from the neural crest lineage. Here we provide array-based methylation data of iNBSCs reprogrammed from adult dermal fibroblasts (ADF), iPSC-derived NBSCs and adult dermal fibroblasts. The data provided demonstrate robust changes in the methylation landscape after reprogramming of human adult dermal fibroblasts into iNBSCs.

2018-12-20 | E-MTAB-5808 | biostudies-arrayexpress

Human cardiac fibroblast membrane proteome

Project description:Cardiac Fibroblasts (CFs) are one of the main cell populations in the heart and play important roles in tissue homeostasis and in myocardial fibrosis. The study of these cells has been hampered by the lack of reliable membrane markers: none of the antigens currently used for characterization and isolation of CFs is unique for this cell type. This issue has also raised doubts regarding a distinct identity of cardiac fibroblasts when comparing to other myocardium cell populations with similar morphologies. In this work, we report a comprehensive description and functional analysis of human CFs (hCFs) enriched membrane fraction proteome by advanced proteomic mass spectrometry-based tools. A total number of 1478 proteins were identified, including a 916 membrane proteins (62%). We also report on the identification of a set of 37 membrane proteins specific for hCFs by direct comparison with proteome lists of human mesenchymal stem cells (hMSCs), human dermal fibroblasts (hDFs) and human cardiac stem cells (hCSCs, hCSCs membrane proteome list is also available in this repository). The data reported in this work is a valuable source of information for further studies aiming at the discovery of a membrane molecular signature of hCFs.

2018-05-11 | PXD007839 | Pride