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Comparison of Partially Denatured Cytochrome c Structural Ensembles in Solution and Gas Phases Using Cross-Linking Mass Spectrometry

ABSTRACT: Datasets in the publication Comparison of Partially Denatured Cytochrome c Structural Ensembles in Solution and Gas Phases Using Cross-Linking Mass Spectrometry

ORGANISM(S): Equus Caballus

SUBMITTER: Ian K Webb

PROVIDER: PXD064154 | JPOST Repository | Thu May 22 00:00:00 BST 2025

REPOSITORIES: jPOST

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			Action	DRS
	02-07-2023_CYTC_70ACN_10BS3_ECD.raw	Raw
	02-27-2023_10CYTC_ACNONLY_ECD_2.raw	Raw
	02-27-2023_7CYTC_ACNONLY_ECD_2.raw	Raw
	02-28-2023_10CYTC_ACN_ECD_1.raw	Raw
	02-28-2023_7CYTC_ACN_ECD_1.raw	Raw

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Publications

Comparison of Partially Denatured Cytochrome <i>c</i> Structural Ensembles in Solution and Gas Phases Using Cross-Linking Mass Spectrometry.

Cain Rebecca L RL Webb Ian K IK

Journal of the American Society for Mass Spectrometry 20241212 1

Electrospray ionization mass spectrometry (ESI-MS) can retain intact protein structures, but details about partially folded and unfolded protein structures during and after introduction to the gas phase are elusive. Here we use ESI-MS with chemical cross-linkers to compare denatured cytochrome <i>c</i> structures in both solution and gas phases. Solution phase cross-linking prior to ESI captures solution phase structures, while gas phase cross-linking through ion/ion reactions in the trap cell c ...[more]

PMID: 39665677

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