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A two-dimensional peptide-centric stability assay maps multidimensional target landscapes unveiling 17Î²-estradiol (E2)-GPX4 binding-induced ferroptosis

ABSTRACT: We developed a two-dimensional (2D) PEptide-centric Local Stability Assay (PELSA) which allows highly reliable target identification (~ 98% true identification for staurosporine) and systematic readout of multidimensional structural effects (direct interaction, allosteric regulation, complex regulation, etc.) induced by increasing ligand concentrations and local binding rate in native cellular environment. We then demonstrated the versatility of 2D-PELSA by investigating the interactions of 17Î²-estradiol (E2), a predominant sex hormone that exerts a wide spectrum function in both health and disease.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Mingliang Ye

PROVIDER: PXD064877 | JPOST Repository | Thu Jun 11 00:00:00 BST 2026

REPOSITORIES: jPOST

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			Action	DRS
	HF_LYN1_20211104_DIA_Try1v2_BT474_100nME2_4.raw	Raw
	HF_LYN1_20211104_DIA_Try1v2_BT474_100nME2_5.raw	Raw
	HF_LYN1_20211104_DIA_Try1v2_BT474_100uME2_1.raw	Raw
	HF_LYN1_20211104_DIA_Try1v2_BT474_100uME2_2.raw	Raw
	HF_LYN1_20211104_DIA_Try1v2_BT474_100uME2_3.raw	Raw

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