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LC-MS/MS-based label-free protein quantification from protein G-bound and unbound fractions of papain-digested IgG

ABSTRACT: The identification of protein G-bound and unbound fractions of papain-digested IgG

ORGANISM(S): Rattus Rattus (black Rat)

SUBMITTER: Hirokazu Yagi

PROVIDER: PXD065199 | JPOST Repository | Mon Oct 20 00:00:00 BST 2025

REPOSITORIES: jPOST

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			Action	DRS
	20250403_dda_ratbound_01.raw	Raw
	20250403_dda_ratunbound_01.raw	Raw
	List%20of%20peptides%20from%20protein%20G-bound%20and%20unbound%20fractions%20of%20papain-digested%20IgG%20.xlsx	Xlsx

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Publications

Temporal and Sex-Dependent N-Glycosylation Dynamics in Rat Serum.

Yagi Hirokazu H Kondo Sachiko S Murakami Reiko R Yogo Rina R Yanaka Saeko S Umezawa Fumiko F Yagi-Utsumi Maho M Fujita Akihiro A Okina Masako M Hashimoto Yutaka Y Hotta Yuji Y Kato Yoichi Y Nakajima Kazuki K Furukawa Jun-Ichi JI Kato Koichi K

International journal of molecular sciences 20250727 15

We conducted systematic glycomic and glycoproteomic profiling to characterize the dynamic N-glycosylation landscape of rat serum, with particular focus on sex- and time-dependent variations. MALDI-TOF-MS analysis revealed that rat serum N-glycans are predominantly biantennary, disialylated complex-type structures with extensive O-acetylation of Neu5Ac residues, especially in females. LC-MS/MS-based glycoproteomic analysis of albumin/IgG-depleted serum identified 87 glycoprot ...[more]

PMID: 40806398

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