Dataset Information

Glycoprpteomics for Albumin & IgG depleted

ABSTRACT: Glycoprpteomics for Albumin & IgG depleted rat serum

ORGANISM(S): Cellular Organisms

SUBMITTER: Hirokazu Yagi

PROVIDER: PXD065209 | JPOST Repository | Mon Oct 20 00:00:00 BST 2025

REPOSITORIES: jPOST

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	230511_yagiRatF1_1_001.raw	Raw
	230511_yagiRatF1_1_002.raw	Raw
	230511_yagiRatM1_3_001.raw	Raw
	230511_yagiRatM1_3_002.raw	Raw
	230512_yagiRatF1_2_001.raw	Raw

Items per page:

1 - 5 of 20

Publications

Temporal and Sex-Dependent N-Glycosylation Dynamics in Rat Serum.

Yagi Hirokazu H Kondo Sachiko S Murakami Reiko R Yogo Rina R Yanaka Saeko S Umezawa Fumiko F Yagi-Utsumi Maho M Fujita Akihiro A Okina Masako M Hashimoto Yutaka Y Hotta Yuji Y Kato Yoichi Y Nakajima Kazuki K Furukawa Jun-Ichi JI Kato Koichi K

International journal of molecular sciences 20250727 15

We conducted systematic glycomic and glycoproteomic profiling to characterize the dynamic N-glycosylation landscape of rat serum, with particular focus on sex- and time-dependent variations. MALDI-TOF-MS analysis revealed that rat serum N-glycans are predominantly biantennary, disialylated complex-type structures with extensive O-acetylation of Neu5Ac residues, especially in females. LC-MS/MS-based glycoproteomic analysis of albumin/IgG-depleted serum identified 87 glycoprot ...[more]

PMID: 40806398

Similar Datasets

Project description:Systemic lupus erythematosus is a chronic autoimmune disease with multifactorial ethiopathogenesis. The complement system is involved in both the early and late stages of disease development and organ damage. To better understand autoantibody mediated complement consumption the GAPAID consortium examined ex vivo immune complex formation on autoantigen arrays. We recruited patients with SLE (n=211), with other systemic autoimmune diseases (n=65) and non-autoimmune control subjects (n=149) in two rheumatology tertiary care centers. Standard clinical and laboratory data were collected from all subjects and serum complement levels were determined in SLE patients. The genotype of SNP rs1143679 in the ITGAM gene was also determined. On-chip formation of immune complexes was examined using a functional immunoassay on autoantigen microarray. The amount of antigen-bound IgM, IgG and complement C4 and C3 was quantified on autoantigens comprising nucleic acids, proteins and lipids. Our results show that the relatively high complement consumption of nucleic acids is further increased upon binding of IgM and IgG. This is true even when serum complement levels are decreased due to complement consumption in SLE patients. A negative correlation between serum complement levels and ex vivo complement deposition on nucleic acid autoantigens is demonstrated. On the contrary, most protein and lipid autoantigens show positive correlation with C4 and C3 levels. Genetic analysis reveals that the non-synonymous variant rs1143679 in complement receptor type 3 is associated with an increased production of anti-dsDNA IgG antibodies. Notwithstanding, homozygous carriers of the previously reported susceptible allele (AA) have lower levels of dsDNA specific IgM among SLE patients. Regarding organ involvement we find that besides anti-C1q IgG, low levels of dsDNA specific IgM and low complement C4 binding to C1q are also associated with renal injury. In summary, nucleic acids maintain a skewed complement deposition balance when bound by IgG and IgM, depleting the early classical complement pathway from other physiological processes. Dysfunction of the receptor responsible for complement-mediated apoptotic debris removal promotes the development of autoantibodies targeting nucleic acids. These observations provide serological and genetic evidence for complement-mediated clearance deficiency of apoptotic debris in lupus.

Dataset Information

Glycoprpteomics for Albumin & IgG depleted

Dataset's files

Publications

Temporal and Sex-Dependent <i>N</i>-Glycosylation Dynamics in Rat Serum.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Glycoprpteomics for Albumin & IgG depleted

Dataset's files

Publications

Temporal and Sex-Dependent &lt;i&gt;N&lt;/i&gt;-Glycosylation Dynamics in Rat Serum.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Temporal and Sex-Dependent <i>N</i>-Glycosylation Dynamics in Rat Serum.