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Proteomics analysis of iPS spheres

ABSTRACT: Proteomics analysis of membrane proteins extracted from iPS spheres. Membrane fractions were isolated, treated with 8 M urea, and trypsinized. The peptides were fractionated (4 fractions) and analyzed by LC-MS/MS (Q-Exactive and UHPLC).

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Shushi Nagamori

PROVIDER: PXD065256 | JPOST Repository | Tue Aug 05 00:00:00 BST 2025

REPOSITORIES: jPOST

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			Action	DRS
	03_EK30_S6END_F0.raw	Raw
	04_EK30_S6END_F1.raw	Raw
	05_EK30_S6END_F2.raw	Raw
	06_EK30_S6END_F3.raw	Raw
	16_EK30_S5END_F0.raw	Raw

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Publications

Zinc dynamics in the pluripotent stem cells during maintenance of pluripotency and pancreatic differentiation.

Shiraki Nobuaki N Kadokura Tamano T Hashiguchi Rena R Sim Erinn Zixuan EZ Kongpracha Pornparn P Wiriyasermkul Pattama P Nagamori Shushi S Arakawa Akihiro A Kume Shoen S

Scientific reports 20260221 1

We previously reported that pluripotent stem cells (PSCs) are highly dependent on methionine metabolism and that S-adenosyl methionine (SAM) acts as a key metabolite in sustaining the pluripotency of PSCs. We reported that zinc signaling is one of the downstream pathways for methionine metabolism and that zinc is crucial in regulating the self-renewal and differentiation of PSCs. An interdependent relationship exists between methionine metabolism and zinc dynamics. Here, we aim to reveal the zin ...[more]

PMID: 41723219

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Gene expression profiles in human iPS cells cultured by different four methods

Project description:Hepatocyte-like cells differentiated from human iPS cells are expected to be utilized in pharmaceutical research and regenerative medicine. Recently, various culture methods for human iPS cell maintenance have been developed. However, it is not well known whether human iPS cell maintenance method affects hepatic differentiation potency. In this study, we cultured human iPS cells using four maintenance methods: ReproStem medium with feeder cells (mouse embryonic fibroblasts), AK02N medium with iMatrix-511 (E8 fragments of laminin511), Essential 8 medium with Vitronectin N (N-terminal domain of vitronectin), TeSR-E8 medium with Vitronectin XF (xeno-free vitronectin). Then, these human iPS cells were differentiated into the hepatocyte-like cells. Interestingly, the gene expression levels of definitive endoderm markers in the definitive endoderm cells generated from human iPS cells cultured with ReproStem or TeSR-E8 medium were higher than those in other groups. The gene expression level of foregut marker, HHEX, in the definitive endoderm cells generated from human iPS cells cultured with ReproStem medium was higher than that in other groups. Consistently, the expression levels of hepatocyte markers, albumin and urea secretion capacity, and CYP3A4 activity in the hepatocyte-like cells generated from human iPS cells cultured with ReproStem medium were higher than those in other groups. Our data indicated that the most suitable human iPS cell maintenance method for efficient hepatic differentiation was the on-feeder method which uses mouse embryonic fibroblasts, but not feeder-free methods. In conclusion, human iPS cell maintenance method largely affects hepatic differentiation potency.

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Modeling SARS-CoV-2 infection and its individual differences with ACE2-expressing human iPS cells

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Expression data throughout reprogramming of MEF to iPS using a Dox-inducible promoter

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LEOPARD Syndrome iPS, BJ iPS and Fibroblasts

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Ips typographus

Project description:Ips typographus overview

| PRJNA191580 | ENA