Proteomics

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VHL-AirID expressing HCC827-Luc cells treated with DMSO, VH032 or MS39

ABSTRACT: HCC827-Luc cells stably expressing VHL-AirID were pretreated with MG132. The DMSO, VH032 or MS39 were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified by LC-MS/MS analysis.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Hidetaka Kosako 

PROVIDER: PXD067144 | JPOST Repository | Wed Sep 03 00:00:00 BST 2025

REPOSITORIES: jPOST

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Dataset's files

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Action DRS
114_PJ1223_Yamada_1_VHL_AirID_DMSO_Tama_QEp.raw Raw
116_PJ1223_Yamada_4_VHL_AirID_VH032_Tama_QEp.raw Raw
118_PJ1223_Yamada_7_VHL_AirID_MS39_Tama_QEp.raw Raw
120_PJ1223_Yamada_2_VHL_AirID_DMSO_Tama_QEp.raw Raw
122_PJ1223_Yamada_5_VHL_AirID_VH032_Tama_QEp.raw Raw
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Publications

In-cell proximity target validation methods for heterobifunctional molecules with CRBN- or VHL-binder using AirID.

Yamada Kohdai K   Yamanaka Satoshi S   Yamakoshi Hiroyuki H   Kohyama Aki A   Iwabuchi Yoshiharu Y   Kosako Hidetaka H   Sawasaki Tatsuya T  

Communications biology 20250830 1

Heterobifunctional molecules, such as proteolysis-targeting and autophagy-targeting chimera, represent new drug concepts. They are composed of two protein binders that can induce proximity interactions between two proteins and protein catalysis. Currently, cereblon (CRBN)- and von Hippel-Lindau (VHL)-binders with thalidomide- and VH032-backbones are widely used as E3 ligase binders. Here, we developed a method to validate proteins that interact with heterobifunctional molecules in cells using Ai  ...[more]

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