Dataset Information

ThBD-AGIA-AirID expressing LNCaP cells treated with Pomalidomide or ARM-110

ABSTRACT: LNCaP cells stably expressing ThBD-AGIA-AirID were pretreated with MG132. Pomalidomide or ARV-110 was added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified by LC-MS/MS analysis.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Hidetaka Kosako

PROVIDER: PXD067147 | JPOST Repository | Wed Sep 03 00:00:00 BST 2025

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	41_PJ1322_Yamada_1_ThBD_AGIA_AirID_Pom_Tama_FusionIT.raw	Raw
	43_PJ1322_Yamada_4_ThBD_AGIA_AirID_ARV110_Tama_FusionIT.raw	Raw
	45_PJ1322_Yamada_2_ThBD_AGIA_AirID_Pom_Tama_FusionIT.raw	Raw
	47_PJ1322_Yamada_5_ThBD_AGIA_AirID_ARV110_Tama_FusionIT.raw	Raw
	49_PJ1322_Yamada_3_ThBD_AGIA_AirID_Pom_Tama_FusionIT.raw	Raw

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Publications

In-cell proximity target validation methods for heterobifunctional molecules with CRBN- or VHL-binder using AirID.

Yamada Kohdai K Yamanaka Satoshi S Yamakoshi Hiroyuki H Kohyama Aki A Iwabuchi Yoshiharu Y Kosako Hidetaka H Sawasaki Tatsuya T

Communications biology 20250830 1

Heterobifunctional molecules, such as proteolysis-targeting and autophagy-targeting chimera, represent new drug concepts. They are composed of two protein binders that can induce proximity interactions between two proteins and protein catalysis. Currently, cereblon (CRBN)- and von Hippel-Lindau (VHL)-binders with thalidomide- and VH032-backbones are widely used as E3 ligase binders. Here, we developed a method to validate proteins that interact with heterobifunctional molecules in cells using Ai ...[more]

PMID: 40885760

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