Proteomic Analysis of Bacterial Culture Supernatants

ABSTRACT: The supernatants from bacterial cultures were collected, concentrated by ultrafiltration (3 kDa cutoff), and subsequently reduced, alkylated, and digested with trypsin. Digested peptides were purified via SCX and StageTips, dried, and resuspended in 5% ACN with 0.1% TFA. LC-MS/MS analysis was performed using an Orbitrap QE Plus.

ORGANISM(S): Cellular Organisms

SUBMITTER: Takumi Nishiuchi 

PROVIDER: PXD067284 | JPOST Repository | Wed Aug 13 00:00:00 GMT+01:00 2025

REPOSITORIES: jPOST