Rapid and Label-Free Structural Proteomics by One-Step Swift Trypsin LiP-MS
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens (human) Cellular Organisms
SUBMITTER: Yusuke Kawashima
PROVIDER: PXD069771 | JPOST Repository | Mon Jan 05 00:00:00 GMT 2026
REPOSITORIES: jPOST
Dataset's files
| Action | DRS | |||
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| 240524_miya-7_Temp4-1.raw | Raw | |||
| 240524_miya-7_Temp4-2.raw | Raw | |||
| 240524_miya-7_Temp4-3.raw | Raw | |||
| 240524_miya-7_Temp4-4.raw | Raw | |||
| 240524_miya-8_Temp4_stop-1.raw | Raw |
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Publications
Rapid and Label-Free Structural Proteomics Using One-Step Swift Trypsin LiP-MS.
Miyashita Yasuomi Y Konno Ryo R Ogasawara Satoshi S Okuda Yusei Y Takamuku Yuuki Y Moriya Toshio T Saito Tetsuichiro T Murata Takeshi T Ohara Osamu O Kawashima Yusuke Y
ACS omega 20251225 1
Limited proteolysis mass spectrometry (LiP-MS) is a powerful approach for probing protein conformational changes on a proteome-wide scale. However, conventional workflows rely on a two-step digestion with proteinase K and trypsin, which increases complexity and reduces reproducibility and sensitivity. This study aimed to develop a simplified one-step protocol, termed Swift Trypsin LiP-MS (STLiP-MS), which uses a trypsin-immobilized spin column and high-speed centrifugation to achieve rapid and r ...[more]
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