Phosphorylation sites of Calpain-3 expressed in HEK293T cells with or without co-expression of constitutively active form of Fyn

ABSTRACT: HEK293T cells were transiently transfected with plasmids encoding Maltose binding protein (MBP)-tagged proteolytically inactive CAPN (CAPN3:CS) and constitutively active form of Fyn (Fyn-YF). CAPN3:CS was pulldown from the HEK293T cell lysates using amylose-resin, purified as an excised band from SDS-PAGE and the phosphorylated sites of CAPN3 were identified by MS/MS analysis. We detected several phosphorylation sites, and phosphorylation at Ser19 was most significant and dependent on Fyn-YF co-expression.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Chihiro Hisatsune 

PROVIDER: PXD076638 | JPOST Repository | Thu Oct 15 00:00:00 BST 2026

REPOSITORIES: jPOST