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The endo-lysosomal system drives lumen formation in a human epiblast model

ABSTRACT: In this study, the endolysosomal dynamics of H9 human embryonic stem cells (hESC) undergoing apicosome formation was examined using a spatial proteomics approach based on the engineered ascorbate peroxidase (APEX) 2, followed by mass spectrometry analysis. In detail, we generated H9 hESC line that express APEX2 fused to Podocalyxin in a doxycycline (DOX) inducible manner, which were then grown in the apicosome forming condition in the presence of DOX. At 30-hr timepoint, proximity biotinylation was performed using our previously established APEX2-labeling strategy, which was followed by TMT labeling and mass spectrometry analysis.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Kenichiro Taniguchi

PROVIDER: PXD079227 | JPOST Repository | Tue Jul 21 00:00:00 GMT+01:00 2026

REPOSITORIES: jPOST

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			Action	DRS
	PODXL-APEX_1_0576.raw	Raw
	PODXL-APEX_2_0577.raw	Raw
	PODXL-APEX_3_0578.raw	Raw
	PODXL-APEX_4_0579.raw	Raw
	PODXL-APEX_5_0580.raw	Raw

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