Pull-down assay coupled with mass spectrometry using Vstm2b-Fc and rat primary hippocampal neurons

ABSTRACT: This study aimed to identify Vstm2b-interacting proteins in rat primary hippocampal neurons cultured for 14 days in vitro. Neurons were incubated with Vstm2b-Fc– or Fc-coated Protein G magnetic beads and crosslinked with DTSSP. Following cell lysis, bound proteins were isolated by magnetic separation and subjected to proteomic analysis.

ORGANISM(S): Rattus Rattus (black Rat)

SUBMITTER: Hideto Takahashi 

PROVIDER: PXD079896 | JPOST Repository | Wed Aug 12 00:00:00 GMT+01:00 2026

REPOSITORIES: jPOST