HCK and FYN kinase domain variants expressed in HEK293T cells

ABSTRACT: HEK293T cells expressing HCK and FYN kinase domain variants fused with N-terminal GFP domain. Cells expressing only GFP were used as control. Cell lysate were trypsinized, collected in 1.5 mL tubes, and prepared for mass spectrometry (MS) analysis. Protein digestion, tryptic peptide purification, and phosphopeptide enrichment were performed using the EasyPep Mini MS Sample Prep Kit (A40006) and the High-Select TiO2‚‚ Phosphopeptide Enrichment Kit (A32993) from Thermo Scientific according to the manufacturer's instructions.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Paola Laurino 

PROVIDER: PXD081561 | JPOST Repository | Thu Oct 22 00:00:00 BST 2026

REPOSITORIES: jPOST