JPST000087 Human Fibroblast cell_aHDF1388-P9 [Reanalysis: JPST000087]
Ontology highlight
ABSTRACT: Proteome analyses of human fibroblast cell line (aHDF1388-P9) were carried out using a two-meter monolithic silica C18 capillary columns without prefractionation. Tryptic peptides from 4 μg cell lysates were directly injected onto the 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min. Triplicate measurements were conducted and the merged results were searched against UniProt DB to identify peptides and proteins. The obtained results were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project. [Original project description]
Project description:Proteome analyses of human fibroblast cell line (Tig120slc-P8) were carried out using a two-meter monolithic silica C18 capillary columns without prefractionation. Tryptic peptides from 4 μg cell lysates were directly injected onto the 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min. Triplicate measurements were conducted and the merged results were searched against UniProt DB to identify peptides and proteins. The obtained results were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project. [Original project description]
Project description:Proteome analyses of human fibroblast cell line (aHDF1419-P10) were carried out using a two-meter monolithic silica C18 capillary columns without prefractionation. Tryptic peptides from 4 μg cell lysates were directly injected onto the 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min. Triplicate measurements were conducted and the merged results were searched against UniProt DB to identify peptides and proteins. The obtained results were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project. [Original project description]
Project description:Proteome analyses of human fibroblast cell line (Tig120slc-P8) were carried out using a two-meter monolithic silica C18 capillary columns without prefractionation. Tryptic peptides from 4 μg cell lysates were directly injected onto the 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min. Triplicate measurements were conducted and the merged results were searched against UniProt DB to identify peptides and proteins. The obtained results were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project.
Project description:Proteome analyses of human fibroblast cell line (aHDF1419-P10) were carried out using a two-meter monolithic silica C18 capillary columns without prefractionation. Tryptic peptides from 4 μg cell lysates were directly injected onto the 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min. Triplicate measurements were conducted and the merged results were searched against UniProt DB to identify peptides and proteins. The obtained results were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project.
Project description:Proteome analyses of human fibroblast cell line (aHDF1388-P9) were carried out using a two-meter monolithic silica C18 capillary columns without prefractionation. Tryptic peptides from 4 μg cell lysates were directly injected onto the 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min. Triplicate measurements were conducted and the merged results were searched against UniProt DB to identify peptides and proteins. The obtained results were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project.
Project description:Proteome analyses of human induced pluripotent stem cell line (201B7-P32) were carried out using a two-meter monolithic silica C18 capillary columns without prefractionation. Tryptic peptides from 4 μg cell lysates were directly injected onto the 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min. Triplicate measurements were conducted and the merged results were searched against UniProt DB to identify peptides and proteins. The obtained results were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project. [Original project description]
Project description:Proteome analyses of human induced pluripotent stem cell line (606A1-P46) were carried out using a two-meter monolithic silica C18 capillary columns without prefractionation. Tryptic peptides from 4 μg cell lysates were directly injected onto the 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min. Triplicate measurements were conducted and the merged results were searched against UniProt DB to identify peptides and proteins. The obtained results were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project. [Original project description]
Project description:Proteome analyses of human induced pluripotent stem cell line (585A1-P55) were carried out using a two-meter monolithic silica C18 capillary columns without prefractionation. Tryptic peptides from 4 μg cell lysates were directly injected onto the 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min. Triplicate measurements were conducted and the merged results were searched against UniProt DB to identify peptides and proteins. The obtained results were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project. [Original project description]
Project description:Proteome analyses of human induced pluripotent stem cell line (414C2-P43) were carried out using a two-meter monolithic silica C18 capillary columns without prefractionation. Tryptic peptides from 4 μg cell lysates were directly injected onto the 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min. Triplicate measurements were conducted and the merged results were searched against UniProt DB to identify peptides and proteins. The obtained results were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project. [Original project description]
Project description:Proteome analyses of human induced pluripotent stem cell line (32R1-P32) were carried out using a two-meter monolithic silica C18 capillary columns without prefractionation. Tryptic peptides from 4 μg cell lysates were directly injected onto the 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min. Triplicate measurements were conducted and the merged results were searched against UniProt DB to identify peptides and proteins. The obtained results were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project. [Original project description]