Proteomics

Dataset Information

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Large-scale Discovery of Substrates of the Human Kinome (RET) [Reanalysis: JPST000508]


ABSTRACT: Kinase -networks are important for cellular signal transduction. Despite tremendous efforts to uncover these signaling pathways, huge numbers of uncharacterized phosphosites still remain in the human proteome. Because of the transient nature of kinase-substrate interactions in vivo, it is almost impossible to identify direct substrates. Here, we present a novel strategy for the rapid and high-throughput discovery for of kinase substrates of kinase using quantitative proteomics. Using 385 purified kinases, we identified a total of 175,574 potential direct kinase substrates of kinases. In addition, we identified novel kinase groups, such as one group containing 30 threonine-directed kinases and another containing 15 serine/threonine/tyrosine kinase sgroup. Surprisingly, we observed that the diversity of substrates for tyrosine kinases was much higher than that for serine-threonine kinases. [Original project description]

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yasushi Ishihama 

PROVIDER: RPXD049615 | JPOST Repository | Wed Feb 21 00:00:00 GMT 2024

REPOSITORIES: jPOST

Dataset's files

Source:
Action DRS
mai100723_014RET-Y791F.maxq.txt Txt
mai100723_014RET-Y791F.txt Txt
rawfiles.txt Txt
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