Project description:ATAC-Seq was carried out on isolated nuclei obtained from induced Pluripotnent Stem Cells (iPSC) cell lines. These lines were derived from ALS, SMA and Control (unaffected) individuals (three of each).
Project description:Total RNA-Seq was carried out on rRNA depleted RNA obtained from motor neuron lines generated from subject induced pluripotent stem cell (iPSC) lines. The iPSC lines were derived from 4 ALS, 3 SMA and 3 Control (unaffected) individuals, in duplicate.
Project description:DIA proteomics was carried out on total protein obtained from motor neuron lines generated from subject induced pluripotent stem cell (iPSC) lines. The iPSC lines were derived from 11 ALS and 4 Control (unaffected) individuals, in duplicate.
Project description:DIA proteomics was carried out on total protein obtained from motor neuron lines generated from subject induced pluripotent stem cell (iPSC) lines. The iPSC lines were derived from 11 ALS and 4 Control (unaffected) individuals, in duplicate.
Project description:ATAC-Seq was carried out on isolated nuclei obtained from induced Pluripotnent Stem Cells (iPSC) cell lines. These lines were derived from ALS, SMA and Control (unaffected) individuals (three of each).
Project description:Total RNA-Seq was carried out on rRNA depleted RNA obtained from motor neuron lines generated from subject induced pluripotent stem cell (iPSC) lines. The iPSC lines were derived from 11 ALS and 4 Control (unaffected) individuals, in duplicate.
Project description:Spinal Muscular Atrophy (SMA) is an autosomal recessive motor neuron disease and is the second most common genetic disorder leading to death in childhood. Motoneurons derived from induced pluripotent stem cells (iPSC) obtained by reprogramming SMA patient and his healthy father fibroblasts, and genetically corrected SMA-iPSC obtained converting SMN2 into SMN1 with target gene correction (TGC), were used to study gene expression and splicing events linked to pathogenetic mechanisms. Microarray technology was used to assess the global gene expression profile as well as splicing events of iPS-derived motorneurons from SMA patient, unaffected father and TGC-treated cells.
Project description:RNA-Seq was carried out on isolated mRNA obtained from induced Pluripotnent Stem Cells (iPSC) cell lines. These lines were derived from ALS, SMA and Control (unaffected) individuals (three of each).