Similar Datasets
Project description:MCF10A cells were grown in standard 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), phalloidin (actin), CellMask (cytoplasm) and MitoTracker (metabolic activity). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.
2016-11-10 | LDS-1443 | LINCS
Project description:MCF10A cells were grown in standard 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), CellMask (cytoplasm) KRT5 (basal lineage) and KRT19 (luminal lineage). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.
2016-11-10 | LDS-1447 | LINCS
Project description:HMEC122L cells were grown in standard 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), KRT5(basal lineage), KRT19(luminal lineage) and EdU(Active S phase). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.
2016-11-10 | LDS-1431 | LINCS
Project description:Cell cycle and nuclear state imaging readouts for PC3 cells treated with ligand and ECMp combinations to explore the role of microenvironmental signals in cancer growth.
2016-03-09 | LDS-1311 | LINCS
Project description:HMEC240L cells were grown in standard 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), phalloidin (actin), CellMask (cytoplasm) and MitoTracker (metabolic activity). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.
2016-11-10 | LDS-1435 | LINCS
Project description:Cell cycle and nuclear state imaging readouts for YAPC cells treated with ligand and ECMp combinations to explore the role of microenvironmental signals in cancer growth.
2016-03-09 | LDS-1327 | LINCS
Project description:Cell cycle and nuclear state imaging readouts for YAPC cells treated with ligand and ECMp combinations to explore the role of microenvironmental signals in cancer growth.
2016-03-09 | LDS-1335 | LINCS