Project description:The response of HepG2-SF cells exposed to 100 µM fatty acids during 7 days was investigated using stable isotope labeling by amino acids in cell culture (SILAC) for analysis of the full proteome
Project description:To measure translational efficiency in FMRP depletion, we purified RNAs from either wild-type or FMR1-knockout (FMR1-KO) SH-SY5Y cells generated for SILAC coupled to LC-MS/MS analysis and performed RNA-seq to quantitate mRNA abundance to normalize their protein abundance.