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Couzens_Hippo_P86_VS5_VS6

ABSTRACT: This submission is part of the mass spectrometry datasets for the manuscript by Couzens et al. This submission contains files for an okadaic acid time course (association of proteins to the MOB1A bait in a phosphorylation dependent manner) and a comparison of the interactions mediated by phosphatase inhibition for the following baits: MOB1A, MOB1B and MOB4 in comparison to a negative control. See the README file within "Methods and Protocols" and the accompanying File description.

PROVIDER: MSV000078449 | MassIVE | Fri Jun 28 00:00:00 BST 2013

REPOSITORIES: MassIVE

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Protein interaction network of the mammalian Hippo pathway reveals mechanisms of kinase-phosphatase interactions.

Couzens Amber L AL Knight James D R JD Kean Michelle J MJ Teo Guoci G Weiss Alexander A Dunham Wade H WH Lin Zhen-Yuan ZY Bagshaw Richard D RD Sicheri Frank F Pawson Tony T Wrana Jeffrey L JL Choi Hyungwon H Gingras Anne-Claude AC

Science signaling 20131119 302

The Hippo pathway regulates organ size and tissue homeostasis in response to multiple stimuli, including cell density and mechanotransduction. Pharmacological inhibition of phosphatases can also stimulate Hippo signaling in cell culture. We defined the Hippo protein-protein interaction network with and without inhibition of serine and threonine phosphatases by okadaic acid. We identified 749 protein interactions, including 599 previously unrecognized interactions, and demonstrated that several i ...[more]

PMID: 24255178

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