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Integrated proteomic analysis of post-translational modifications by serial enrichment.

ABSTRACT: Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA. Nat Methods 2013, 10, 634-7. doi: 10.1038/nmeth.2518.

PROVIDER: MSV000078509 | MassIVE | Tue Dec 10 20:06:00 GMT 2013

REPOSITORIES: MassIVE

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Integrated proteomic analysis of post-translational modifications by serial enrichment.

Mertins Philipp P Qiao Jana W JW Patel Jinal J Udeshi Namrata D ND Clauser Karl R KR Mani D R DR Burgess Michael W MW Gillette Michael A MA Jaffe Jacob D JD Carr Steven A SA

Nature methods 20130609 7

We report a mass spectrometry-based method for the integrated analysis of protein expression, phosphorylation, ubiquitination and acetylation by serial enrichments of different post-translational modifications (SEPTM) from the same biological sample. This technology enabled quantitative analysis of nearly 8,000 proteins and more than 20,000 phosphorylation, 15,000 ubiquitination and 3,000 acetylation sites per experiment, generating a holistic view of cellular signal transduction pathways as exe ...[more]

PMID: 23749302

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