Lambert_P82_VS4_Chromatin_BioID_APMS_12JUL2014

ABSTRACT: This submission contains the mass spectrometry files for the manuscript by Lambert et al. that describes the use of BioID for chromatin associated protein complexes. A detail comparison to a chromatin optimized AP-MS protocol was also performed. This submission contains 39 IDA files for the following baits: empty 3XFLAG control, GFP-BirA-FLAG control, NLS-BirA-FLAG control, H2B-BirA-FLAG, H3.1-BirA-FLAG, 3XFLAG-MED4, BirA-FLAG-MED4, MED20-3XFLAG, MED20-BirA-FLAG, 3XFLAG-MED23 and BirA-FLAG-MED23. Two biological replicates for every bait were analyzed by the standard Gingras lab BioID protocol and by a protocol optimized for chromatin associated baits (AP-MS). See the README file within "Methods and Protocols" and the accompanying File description. Additional details and files are found on prohits-web.lunenfeld.ca.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anne-Claude Gingras 

PROVIDER: MSV000078782 | MassIVE | Thu Jul 17 10:44:00 BST 2014

REPOSITORIES: MassIVE