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BDNF BONLAC in HEK cells

ABSTRACT: In-depth Quantitative Proteomic Analysis of De Novo Protein Synthesis Induced by Brain-derived Neurotrophic Factor

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Thomas Neubert

PROVIDER: MSV000078831 | MassIVE | Tue Aug 26 09:33:00 BST 2014

REPOSITORIES: MassIVE

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Publications

In-depth quantitative proteomic analysis of de novo protein synthesis induced by brain-derived neurotrophic factor.

Zhang Guoan G Bowling Heather H Hom Nancy N Kirshenbaum Kent K Klann Eric E Chao Moses V MV Neubert Thomas A TA

Journal of proteome research 20141013 12

Measuring the synthesis of new proteins in the context of a much greater number of pre-existing proteins can be difficult. To overcome this obstacle, bioorthogonal noncanonical amino acid tagging (BONCAT) can be combined with stable isotope labeling by amino acid in cell culture (SILAC) for comparative proteomic analysis of de novo protein synthesis (BONLAC). In the present study, we show that alkyne resin-based isolation of l-azidohomoalanine (AHA)-labeled proteins using azide/alkyne cycloaddit ...[more]

PMID: 25271054

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