Project description:Several oral bacterial species were grown anaerobically and fed either glucose or lactate in SHI medium (for method see: Edlund et al., 2013, Edlund et al, 2015 - see http://www.microbiomejournal.com/content/pdf/2049-2618-1-25.pdf & http://www.nature.com/ismej/journal/vaop/ncurrent/full/ismej201572a.html). Biofilm samples for LCMS were collected at different time points from growth wells. After growth was established in SHI medium, biofilms were washed with minimal medium and supplemented with either glucose or lactate (Veillonella species) (see Edlund et al., 2013, Edlund et al., 2015). Samples were collected both after the initial biofilm establishment in SHI medium and after the addition of the supplemented cdm medium. According to gnps network analyses and data sorting exercises: 1) ~10% of this data is reproducible; 2) several of the present masses can also be detected both in SHI and cdm medium. mzXML files for media is not uploaded here (i.e. no media subtraction can be employed using these files only).
Project description:The purpose of this study was to isolate NCSCs from oral mucosa using the neurosphere technique. Total RNA from human oral mucosa stromal cells and sphere-formig oral mucosa stromal cells was collected and compared at their gene expression level. Samples from 3 patients were analysed.
Project description:To investigate the virological properties of SARS-CoV-2 variants, we amplified the clinical isolates of an early pandemic D614G-bearing isolate (B.1.1 lineage, strain TKYE610670; GISAID ID: EPI_ISL_479681), a Delta isolate (B.1.617.2 lineage, strain TKYTK1734; GISAID ID: EPI_ISL_2378732) and an Omicron isolate (BA.1 lineage, strain TY38-873; GISAID ID: EPI_ISL_7418017) and prepared the working viruses.
Project description:Our group recently transcriptomically characterized coculture growth between Streptococcus mutans and several species of commensal streptococci (Rose et al, 2023). However, these experiments were carried out in our lab-based experimental medium, tryptone and yeast extract (TY-). To understand whether culturing these species within a medium that more closely mimics their natural environment alters the interaction, we evaluated both monoculture and coculture growth between the dental caries pathogen Streptococcus mutans and oral commensal species Streptococcus oralis in a half TY- / half human saliva mix that was optimally chosen based on our initial characterization of oral streptococci behaviors in medium mixes containing saliva. Our results surprising show that inclusion of saliva enhances the competition of Streptococcus mutans against commensal streptococci through upregulation of carbohydrate uptake and glycolytic pathways.