Age-related changes in protein abundance in nematodes

ABSTRACT: Using quantitative mass spectrometry together with protein and peptide-level fractionation, we can now report a major increase in coverage of the C. elegans proteome, identifying over 9,300 proteins in three biological replicates. The resulting data have been assembled into a searchable, online resource with a user-friendly graphical interface to provide convenient and open access to the community (https://www.peptracker.com/epd/). By quantifying these proteins in fertile, post-reproductive and aging animals, we show that there is a striking change in abundance of ~8% of the proteome during the course of aging. Network analysis of our proteomics data reveals a concerted down-regulation of proteins involved in fatty acid, amino acid and carbohydrate metabolism, as well as peroxisome function, with increasing age.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Caenorhabditis Elegans (ncbitaxon:6239)

SUBMITTER: Angus Lamond 

PROVIDER: MSV000079263 | MassIVE | Fri Aug 21 13:37:00 BST 2015

SECONDARY ACCESSION(S): PXD004584

REPOSITORIES: MassIVE