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Mouse adipocyte phosphoproteome during differentiation

ABSTRACT: Kosaku Shinoda, Kana Ohyama, Yutaka Hasegawa, Hsin-Yi Chang, David W. Scheel, Louis Z. Sharp, Haemin Hong, Takashi Hosono, Mayu Ogura, Ayaka Sato, Yasushi Ishihama, and Shingo Kajimura. Phosphoproteomics Identifies CK2 As a Negative Regulator of Beige Adipocyte Thermogenesis and Energy Expenditure. Cell Metabolism: 22(6):997-1008.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Yasushi Ishihama

PROVIDER: MSV000079278 | MassIVE | Fri Aug 28 03:25:00 BST 2015

SECONDARY ACCESSION(S): PXD002812

REPOSITORIES: MassIVE

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Phosphoproteomics Identifies CK2 as a Negative Regulator of Beige Adipocyte Thermogenesis and Energy Expenditure.

Shinoda Kosaku K Ohyama Kana K Hasegawa Yutaka Y Chang Hsin-Yi HY Ogura Mayu M Sato Ayaka A Hong Haemin H Hosono Takashi T Sharp Louis Z LZ Scheel David W DW Graham Mark M Ishihama Yasushi Y Kajimura Shingo S

Cell metabolism 20151108 6

Catecholamines promote lipolysis both in brown and white adipocytes, whereas the same stimuli preferentially activate thermogenesis in brown adipocytes. Molecular mechanisms for the adipose-selective activation of thermogenesis remain poorly understood. Here, we employed quantitative phosphoproteomics to map global and temporal phosphorylation profiles in brown, beige, and white adipocytes under β3-adrenenoceptor activation and identified kinases responsible for the adipose-selective phosphoryla ...[more]

PMID: 26525534

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