Project description:In order to study how mitochondrial proteases function on mouse hematopoietic stem cells, the article constructed Afg3l2 knockout mice. When Afg3l2 was knocked out, mouse hematopoietic stem cells showed HSC depletion accompanied by amino acid accumulation and TCA overactivation. We performed transcriptome and proteome sequencing analysis on cells from wild-type (WT) and Afg3l2-KO mice, and differential gene analysis revealed the Mmadhc protein. Further verification showed that Mmadhc is a substrate of the mitochondrial protease Afg3l2, and overexpression of Mmadhc can mimic the amino acid accumulation and TCA overactivation caused by Afg3l2 knockout. Overall, the mitochondrial protease Afg3l2 affects the homeostasis of hematopoietic stem cells by regulating the level of Mmadhc.
Project description:We sequenced mRNA from from four segments along a developing 9 day old third maize leaf of WT W22 accession and ZmDCT2 KO (in W22 background - dct2-1::Ac) plants, to identify changes in gene expression in dct2-1::Ac as a response to the loss of ZmDCT2 function.
Project description:Cytokines secreted by WT and fascin1 KO BM-DCs maturated by LPS were determined by RayBio Quantibody mouse Th17 array1. Fascin1 KO BM-DCs show reduced secretion of several cytokines including IL-6.
Project description:Data-independent acquisition (DIA) of four replicates of mouse brain tissue lysate for each of the four conditions Gasdermin-D WT, Gasdermin-D KO, Gasdermin-D WT treated with cuprizone, and Gasdermin-D KO treated with cuprizone.