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LC-MS/MS analysis of B12 treated SH-SY5Y cells

ABSTRACT: B12 is a compound that has neuroprotective effects. We investigated the protein expression profile associated with the neuroprotective effects of B12 on human neuronal cells SH-SY5Y using label-free quantitative proteomics.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Lijun Zhong, Juntuo Zhou, Yuxin Yin, Yan Pan

PROVIDER: MSV000079437 | MassIVE | Tue Dec 29 22:41:00 GMT 2015

SECONDARY ACCESSION(S): PXD003391

REPOSITORIES: MassIVE

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Quantitative proteomics study of the neuroprotective effects of B12 on hydrogen peroxide-induced apoptosis in SH-SY5Y cells.

Zhong Lijun L Zhou Juntuo J Chen Xi X Lou Yaxin Y Liu Dan D Zou Xiajuan X Yang Bin B Yin Yuxin Y Pan Yan Y

Scientific reports 20160308

B12 belongs to the coumarin class of compounds that have been shown to have various physiological and pharmacological activities including anti-inflammatory, antibacterial, and antioxidant. In the present study, we characterised the neuroprotective effects of B12 against H2O2-induced neuronal cell damage in SH-SY5Y cells. Protein expression profiling in combination with pathway analysis was deployed to investigate the molecular events associated with the neuroprotective effects in human neuronal ...[more]

PMID: 26951766

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