Efficient micro-scale basic reverse phase peptide fractionation for global and targeted proteomics

ABSTRACT: This work describes a micro-scale basic reverse phase liquid chromatographic (bRPLC) fractionation method that offers high reproducibility and efficiency for peptide mixtures from small (5-20 g) samples. Shotgun and targeted proteomic analysis were performed to detect differentially expressed proteins from lung tumor cell lines that are sensitive (11-18) and resistant (11-18R) to the tyrosine kinase inhibitor erlotinib using micro-bRPLC.

INSTRUMENT(S): LTQ Orbitrap Elite, Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Daniel Liebler 

PROVIDER: MSV000079500 | MassIVE | Tue Feb 09 09:47:00 GMT 2016

SECONDARY ACCESSION(S): PXD003604

REPOSITORIES: MassIVE