Digital mapping of immunopeptidomes

ABSTRACT: We present a novel mass spectrometry-based high-throughput workflow and an open-source computational and data resource to reproducibly identify and quantify HLA-associated peptides. Collectively, the resources support the generation of HLA allele-specific peptide assay libraries consisting of consensus fragment ion spectra, and the analysis of quantitative digital maps of HLA peptidomes generated from a range of biological sources by SWATH mass spectrometry (MS). This study represents the first community-based effort to develop a robust platform for the reproducible and quantitative measurement of the entire repertoire of peptides presented by HLA molecules, an essential step towards the design of efficient immunotherapies.

INSTRUMENT(S): LTQ Orbitrap, TripleTOF 5600, LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr Ruedi Aebersold 

PROVIDER: MSV000079623 | MassIVE | Mon Apr 04 01:32:00 BST 2016

SECONDARY ACCESSION(S): PXD001872

REPOSITORIES: MassIVE