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Application of 193 nm UVPD for phosphoproteomic analysis of HeLa and HCC70

ABSTRACT: Application of 193 nm ultraviolet photodissociation (UVPD) for phosphoproteomics analysis of IMAC enriched HeLa and HCC70 lysates. HCC70 cells were either maintained in full media or serum starved for 6 hours prior to lysis. Each sample was analyzed by three UVPD and three HCD technical replicates using an Orbitrap Fusion mass spectrometer equipped with a Coherent 193 nm excimer laser. Using UVPD, a/x, b/y, and c/z ions were generated and improved phosphate retention was observed on product ions.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Jennifer Brodbelt

PROVIDER: MSV000079626 | MassIVE | Mon Apr 04 20:44:00 BST 2016

SECONDARY ACCESSION(S): PXD003904

REPOSITORIES: MassIVE

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193 nm Ultraviolet Photodissociation Mass Spectrometry for Phosphopeptide Characterization in the Positive and Negative Ion Modes.

Robinson Michelle R MR Taliaferro Juliana M JM Dalby Kevin N KN Brodbelt Jennifer S JS

Journal of proteome research 20160726 8

Advances in liquid chromatography tandem mass spectrometry (LC-MS/MS) have permitted phosphoproteomic analysis on a grand scale, but ongoing challenges specifically associated with confident phosphate localization continue to motivate the development of new fragmentation techniques. In the present study, ultraviolet photodissociation (UVPD) at 193 nm is evaluated for the characterization of phosphopeptides in both positive and negative ion modes. Compared to the more standard higher energy colli ...[more]

PMID: 27425180

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