Proteomics

Dataset Information

49

Urinary polypeptide ETD/CID analysis, part 1


ABSTRACT: Urine as a biofluid is commonly used in clinical diagnostics, including those performed during pregnancy. Urine is a rich source of polypeptides and protein degradation products, which have been filtered from blood plasma, thus urine has strong potential as a source for novel clinical diagnostics in disease. In this study, we examine the urinary peptidome from normal healthy women during pregnancy, to demonstrate that peptides are readily observed. We utilise the dissociation method, electron transfer dissociation (ETD) to increase the identification rate of the peptides present within these samples, as the polypeptide species observed in these samples are large and highly charged. An increase in the number of peptides whose identities could be ascribed using routine database searching methods was enabled via the use of ETD.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo sapiens  

SUBMITTER: Dr Sarah Hart 

PROVIDER: MSV000079920 | MassIVE | Tue Jul 19 13:22:00 BST 2016

SECONDARY ACCESSION(S): PXD002312

REPOSITORIES: MassIVE

Dataset's files

Source:
Action DRS
MSV000079920 Other
params.xml Xml
T9082106.mzML Mzml
T9082106_ITMSms2cid.txt (F001733).mzTab Mztab
T9082106_ITMSms2etd.txt (F001734).mzTab Mztab
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