Dataset Information

Multi-omics approach to identify age-related changes in mesenchymal stem cells

ABSTRACT: Mesenchymal stem cells (MSC) are bone-marrow derived cells, capable of multipotent differentiation into connective tissues including bone, tendon and cartilage. They are an attractive source for autologous cell-based treatments for a range of clinical diseases and injuries. MSCs have been demonstrated to possess an age-related loss of cellular functions including differentiation potential and proliferation capacity; with implications for stem cell therapies in older patients. Furthermore the reduction in differentiation potential could contribute to ageing and age-related disease. Biological aging is coupled with a progressive reduction in the regulation of cellular, tissue and organ interaction, resulting in senescence. The purpose of this study was to investigate the epigenetic, RNA and protein changes in ageing MSCs in order to understand the age-related functional and biological changes required for their applications in regenerative medicine.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr Mandy J Peffers

PROVIDER: MSV000079981 | MassIVE | Fri Jul 22 15:45:00 BST 2016

SECONDARY ACCESSION(S): PXD001952

REPOSITORIES: MassIVE

ACCESS DATA

Publications

Age-related changes in mesenchymal stem cells identified using a multi-omics approach.

Peffers M J MJ Collins J J Fang Y Y Goljanek-Whysall K K Rushton M M Loughlin J J Proctor C C Clegg P D PD

European cells & materials 20160208

Mesenchymal stem cells (MSC) are capable of multipotent differentiation into connective tissues and as such are an attractive source for autologous cell-based treatments for many clinical diseases and injuries. Ageing is associated with various altered cellular phenotypes coupled with a variety of transcriptional, epigenetic and translational changes. Furthermore, the regeneration potential of MSCs is reduced with increasing age and is correlated with changes in cellular functions. This study us ...[more]

PMID: 26853623

Similar Datasets

Project description:Objective: Craniofacial bone defects caused by injuries and congenital diseases are a formidable challenge to clinicians. Research has shown promise in using bone marrow mesenchymal stem cells (BM-MSCs) from limb bones for craniofacial bone regeneration; yet little is known about the potential of BM-MSCs from craniofacial bones. This study compared BM-MSCs isolated from limb and craniofacial bones in pigs, a preclinical model closely resembling humans. Design: Bone marrow was aspirated from the tibia and mandible of four-month-old pigs (n=4), followed by BM-MSC isolation, culture-expansion and confirmation by flow cytometry. Proliferation rates were compared using population doubling times. Osteogenic differentiation was evaluated by quantifying alkaline phosphatase (ALP) activity. Total mRNA was extracted from freshly isolated BM-MSCs and analyzed to compare gene expressions of tibial and mandibular BM-MSCs using an Affymetrix GeneChip porcine genome array, followed by real-time RT-PCR evaluation of two neural crest markers. Results: BM-MSCs from both locations expressed MSC markers without expression of hematopoietic markers. Mandibular BM-MSCs proliferated significantly faster than tibial BM-MSCs. Without osteogenic inducers, mandibular BM-MSC alkaline phosphatase activities were 3.3-fold greater than those of tibial origin. Microarray analysis identified 383 differentially expressed genes in mandibular and tibial BM-MSCs, including higher expression of cranial neural crest-related genes nestin and BMP-4 in mandibular BM-MSCs, a trend also confirmed by real-time RT-PCR. Among differently expressed genes, only 47 showed greater than 1.5-fold differences in expression. Conclusions: These data indicate that despite many similarities in gene expression, mandibular BM-MSCs express of number of genes differently than tibial BM-MSCs and have a phenotypic profile that may make them advantageous for craniofacial bone regeneration. Bone marrow was aspirated from the mandibular symphyseal region and the tibia of 3 pigs. Mesenchymal stem cells were isolated from the bone marrow and cultured to 80% confluence. Cells were harvested for total RNA extraction and the RNA was analyzed by Affymetrix GeneChip porcine genome array.

Dataset Information

Multi-omics approach to identify age-related changes in mesenchymal stem cells

Publications

Age-related changes in mesenchymal stem cells identified using a multi-omics approach.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure